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Solid Phase Peptide Synthesis Capping Procedure

Standard Capping Procedure

  • Filter and wash the resin several times with DMF.
  • Suspend the resin in a DMF solution containing acetic anhydride (50 equivalent based on resin substitution) and pyridine (50 equivalents based on resin substitution).  DIPEA may be substituted for the pyridine.
  • Gently shake at room temperature for 30 minutes.
  • Filter and wash the resin with DMF.
  • Perform a Kaiser test.  If the Kaiser test is not negative, repeat the capping procedure.