AAPPTec General Procedures, Protocols, and Technical Bulletins for Solid Phase Peptide Synthesis
These are recommended general procedures for preparing peptides by solid phase methods utilizing AAPPTec products.
Peptide Synthesis Resins, Loading and Cleavage Procedures
- 1035 – Aminomethyl Resin
- 1039 – Carboxypolystyrene
- 1027 – 2-Chlorotritylchloride Resin for Protected Peptide Synthesis
- 1029 – Amino Acid-2-Chlorotrityl Resin for Peptide Synthesis
- 1175 – DHP Resin for Peptide Synthesis of Peptide Alcohols
- 1042 – Hydroxymethylpolystyrene
- 1046 – Knorr-2-Chlorotrityl Resin for Solid Phase Peptide Synthesis
- 1047 – MBHA Resin
- 1048 – Merrifield Resin for Peptide Synthesis With Boc Chemistry
- 1049 – Oxime Resin
- 1051 – PAM Resin for Peptide Synthesis with Boc-Amino Acids
- 1054 – Fmoc-Rink Amide AM Resin for Peptide Synthesis of Peptide Amidedes
- 1092 – Sieber Amide Resin for Protected Peptide Amide Synthesis
- 1073 – Wang Resin for Peptide Synthesis with Fmoc-Amino Acids
- 1034 – Fmoc-Amino Acid Wang Resins
- 1113 – HMPA Resin Preparation, Coupling and Cleavage
- 1183 – Preparing Low-Loading Fmoc-Amino Acid Wang Resins From High Substitution Wang Resin
- 1074 – Weinreb Resin for Solid Phase Synthesis of Peptide Aldehydes
- 1156 – Use of Polystyrene Resins in Continuous Flow Peptide Synthesizers
- 1198 – Resin Loading Measurement by Fmoc Cleavage
Amino Acid and Peptide Deprotection Procedures
General Protocols
- 1163 – Removal of Boc Protecting Group
- 1140 – Standard Fmoc Deprotection-Coupling Procedure for Peptide Synthesis
- 1173 – Fmoc Removal with DBU in Solid Phase Pepticde Synthesis
- 1162 – Standard Removal of Z Protecting Group in Peptide Synthesis
- 1144 – Removal of Allyl-based Protecting Groups; Aloc and Allyl Esters
- 1207 – Removal of t-Butyl-based Protecting Groups in Solution
- 1178 – Glycopeptide Synthesis and Deprotection
Arginine
- 1026 – Fmoc-Arg(Pmc)-OH vs Fmoc-Arg(Pbf)-OH in Peptide Synthesis
- 1087 – Cleaving Mtr Group from Arginine
Asparagine/Glutamine
Cysteine
- 1123 – Removal of S-Acetamidomethyl Groups
- 1180 – Cys(Acm) Deprotection and On-Resin Peptide Cyclization
- 1124 – Removal of S-Benzyl, S-p-Methylbenzyl, and S-p-Methoxybenzyl Groups
- 1164 – Deprotection of Cys(4-MeBzl)
- 1125 – Removal of S-t-Butyl Groups
- 1127 – Removal of S-Trityl Groups
- 1128 – Removal of S-Fluorenylmethyl Groups
Histidine
Lysine
- 1169 – Removal of 2-Cl-Z
- 1182 – Removal of Dde and ivDde Protecting Groups
- 1181 – Selective Removal of Mtt Protecting Group From Amines
Methionine
Trytophan
Tyrosine
Peptide Coupling Protocols
- 1147 – Coupling Dialkylglycines
- 1177 – Coupling to N-Methyl Amino Acids
- 1179 – Fmoc-AA-OPfp Ester Coupling
- 1095 – BOP (Castro’s Reagent)
- 1096 – Benzotriazole-1-yloxy-tris-pyrrolidinophosphonium Hexafluorophosphate (PyBOP)
- 1102 – Bromo-tris-pyrrolidino-phosphonium hexafluorophosphate (PyBrOP)
- 1170 – DEPBT [3-(Diethylphosphoryloxy)-1,2,3-benzotriazin-4(3H)-one]
- 1104 – Dicyclohexylcarbodiimide (DCC)
- 1105 – Diisopropylcarbodiimide (DIC)
- 1098 – HOBt (N-Hydroxybenzotriazole)
- 1099 – EDC (Water-Soluble Carbodiimide)
- 1205 – HATU
- 1100 – HBTU
- 1206 – HCTU
- 1171 – TDBTU (O-3,4-Dihydro-4-oxo-1,2,3-benzotriazine-3yl-1,1,3,3-tetramethyluronium tetrafluoroborate)
- 1101 – TBTU
- 1103 – N,N,N’,N’-Tetramethyl(succinimido)uronium Tetrafluoroborate (TSTU)
- 1196 – Activation and Coupling of Biotin
- 1202 – Phosphoamino Acids
- 1208 – Methylated Lysines
- 1217 – Glycolic acid
Solid Phase and Solution Phase Test Protocols
- 1188 – Kaiser Test (Ninhydrin Test)
- 1189 – Tests for Unprotected Proline
- 1191 – General Tests for Free Amines
- 1186 – Determining Chiral Purity of Amino Acids
- 1190 – Ellman’s Test
Solid Phase Peptide Synthesis Cleavage Cocktails
- 1192 – “Odorless” TIS Cleavage Cocktail (Reagent B)
- 1157 – Cleavage Cocktail for Methionine Containing Peptides (Reagent H)
- 1193 – Cleavage Cocktail for Peptides Containing Cys, Met, Trp and Tyr Residues (Reagent K)
- 1167 – Low Odor Cleavage Cocktail (Reagent L)
- 1168 – Cleavage Cocktail for Peptide Containing Arg Residues (Reagent R)
Peptide Purification and Handling
- 1172 – HPLC Purification of Peptides
- 1194 – Storage and Handling of Peptides
- 1195 – Dissolving Purified Peptides
- 1085 – Removing Trifluoroacetic Acid (TFA) From Peptides
Additional Technical Information
- 1114 – Fmoc-Rink Amide Linker
- 1121 – PNA Synthesis
- 1137 – Converting CHA and DCHA Salts to Free Acids
- 1176 – Ring Closing Metathesis and Hydrogenation on Resin
- 1184 – Attaching Boc Protecting Group With Boc-ON
- 1185 – Reduction of Disulfide Bonds
- 1197 – Click Chemistry on Resin
- 1203 – On-Resin Peptide Labeling with FITC
- 1204 – On-Resin Peptide Labeling with FAM
- 1209 – Allyl Ester Deprotection and On-Resin Cyclization
