EDC HCl [25952-53-8]

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, EDAC, EDCI, Water-soluble cabodiimide.

Molecular Weight: 191.7

EDC HCl activates carboxylic acids to form amides and esters.  It is soluble in most aqueous and organic solvents and the urea byproduct is also water-soluble.  Isolation of the amide or ester product in solution phase synthesis can thus be achieved by simple water-organic solvent extraction. Utilized with N-hydroxysuccinimide (NHS), EDC canbe employed to conjugate smaller molecules, such as small peptides or fluorescent tags to larger biomolecules in aqueous solution, including proteins, DNA/RNA, and antibodies.

Carbodiimide Mechanism

Coupling Protocol