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Dissolving Purified Peptides

  • 1.Dissolve basic peptides in a small amount of 30% acetic acid, then dilute to the required concentration.
  • 2.Dissolve acidic peptides in a small amount of 10% ammonium bicarbonate, then dilute to the required concentration.
  • 3.For hydrophobic peptides that do not dissolve in water, dissolve the peptide in the minimum amount of dimethylsulfoxide (DMSO) then dilute with water to the required concentration.
  • 4.Peptides that aggregate can be dissolved by adding 6 M urea, 6 M urea with 20% acetic acid or 6 M guanidine hydrochloride salt, then diluting to the required concentration.