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Custom Polyclonal Antibody Production

Custom Polyclonal Antibodies

Featured Polyclonal Antibody Production Services

Antigen Preparation (Polyclonal Antibody Production)

  • Peptide design
  • Peptide synthesis
  • Peptide conjugation
  • Protein expression
  • Custom Polyclonal Antibodies
  • Application guaranteed antibody (ELISA, Western blot, IHC)
  • Modification-specific antibody (phosphorylated, methylated, acetylated)


Antibody Purification (Polyclonal Antibodies)


Antibody Identification (Polyclonal Antibodies)

  • ELISA
  • Dot blot
  • Western blot
  • Immunohistochemistry (IHC)

Antibody Labeling (Polyclonal Antibodies)

  • Horseradish peroxidase (HRP)
  • Biotin

AAPPTec offers a variety of polyclonal antibody production packages. These packages cover all antibody production steps including antigen preparation, immunization, sera collection and antibody identification.

Custom Polyclonal Antibody Production Services

Peptide Services

Time

Peptide Design

1-2 days

Peptide Synthesis

2 weeks

Peptide Conjugation (KLB/BSA/OVA, 5 mg or less)

2-3 days

   

Protein Services

Time

Gene Synthesis

3-4 weeks

Gene Amplification (Template provided by customer, 1000 bp or less)

2-3 weeks

Vector Construction

2-3 weeks

Recombinant Selection

1 week

Protein Expression and Purification

2-3 weeks

   

Polyclonal Antibody Services

Time

Protein Checked by SDS-PAGE

2-3 days

Pre-immune Sera Collection

1 day

Immunization and Sera Collection (2 rabbits)

10 weeks

Immunization and Sera Collection (2 rabbits, fast)

6-8 weeks

ELISA Identification

2-3 days

Prescreen(ELISA)

2-3 days

Prescreen (Western Blot)

1 week

Prescreen (Dot Blot)

1 week

Additional Rabbit (1 rabbit)

6-10 weeks

Animal Maintenance (1 rabbit)

1 week

Additional Bleed (10 mL, 1 rabbit)

1 week

Extension Boost and Bleed (10 mL, 1 rabbit)

1 week

Immunization and Sera Collection (1 goat)

20 weeks

Immunization and Sera Collection (2 rats)

10 weeks

Immunization and Sera Collection (4 mice)

10 weeks

Immunization and Sera Collection (2 guinea pigs)

11 weeks

 

Go To Custom Polyclonal Antibody Production Packages

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