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Slow Gradient HPLC for Peptide Purification

Harris, Lee and Brimble reported in the J. Pept. Sci. (J. Pept. Sci. 2012, 18(9); 549-55.)  that they could purify hundreds of milligrams of peptide on a semi-preparative column in a single step by using a slow gradient of 0.1%B per minute. Their results are...

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Cysteine Racemization

JH Park, et al. reported in J Pept Sci. 2012 ;18(7):442-8 that in the synthesis of Protoxin II, they saw up to 50% racemization of Cys during coupling when they used N-methylmorpholine.  They could significantly reduce racemization if they used 2,4,6-collidine in...

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Hello scientists!

Welcome to Peptide Central where you can ask questions relating to peptides and peptide synthesis, read recent reports about peptides and share your experience with other peptide users and peptide preparers.

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