Anticancer activities of an antimicrobial peptide derivative of Ixosin-B amide.
Hsiao YC, Wang KS, Tsai SH, Chao WT, Lung FD., Bioorg Med Chem Lett. 2013, 23, 5744-7. doi: 10.1016/j.bmcl.2013.07.063. Epub 2013 Aug 9.
In nature, antimicrobial peptides (AMPs) represent the first line of defense against infection by pathogens; thus, they are generally good candidates for the development of antimicrobial agents. Recently, we reported two potent antimicrobial peptides, KWLRRVWRWWR-amide (MAP-04-03) and KRLRRVWRRWR-amide (MAP-04-04), which were derived from a fragment of Ixosin-B-amide (KSDVRRWRSRY). Since some cationic AMPs exhibited cytotoxic activity against cancer cells, in the current study, we further investigated the anticancer activity of these potent antimicrobial peptides by antiproliferative assays and wound-healing assays, and the effect of peptide on the cytoskeleton alteration and cell morphology were analyzed by confocal microscopy. Results indicated that MAP-04-03 not only exhibited inhibitory effects on the proliferation (IC50=61.5Î¼M) and on the cell migration of MCF-7 breast cancer cells (at a concentration of 5Î¼M), but also affected the cytoskeleton at the concentration of 25Î¼M. These results demonstrated that MAP-04-03 can serve as a lead peptide analog for developing potent anticancer agents.
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