A defensin-like peptide from Phaseolus vulgaris cv. ‘King Pole Bean’.
Wong JH, Ip DC, Ng TB, Chan YS, Fang F, Pan WL., Food Chem., 2012, 135, 408-14. Epub 2012 Apr 30. DOI: 10.1016/j.foodchem.2012.04.119
A 5447Da antifungal peptide with an N-terminal sequence highly homologous to plant defensins was purified from Phaseolus vulgaris cv. ‘King Pole Bean’ by anion-exchange chromatography on Q Sepharose and FPLC-gel filtration on Superdex 75. The isolated peptide inhibited growth of a number of fungal species, including Mycosphaerella arachidicola, Saccharomyces cerevisiae and Candida albicans, with IC(50) values of 3.9, 4.0 and 8.4Î¼M, respectively. Using the membrane non-permeable DNA-binding dye SYTOX green, it was found that the peptide increased the cell membrane permeability of M. arachidicola, S. cerevisiae and C. albicans.
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