Harris, Lee and Brimble reported in the J. Pept. Sci. (2012, 18, 1935-42″ href=”http://onlinelibrary.wiley.com/doi/10.1002/psc.2432/abstract” target=”_blank”>J. Pept. Sci. 2012, 18(9); 549-55.) that they could purify hundreds of milligrams of peptide on a semi-preparative column in a single step by using a slow gradient of 0.1%B per minute. Their results are impressive, but will finding the optimum gradient be time consuming?
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